The following is applicable to you if your Windows environment is TLS 1.2 only.
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Requirements for a TLS 1.2 only environment

If your Windows environment is TLS 1.2 only then in your deployment of Spotlight Enterprise ensure the following is true for all Spotlight Clients, Spotlight Diagnostic Server, monitored operating systems, monitored SQL Server instances and .Net.

Ensure the most recent service packs are installed for both the Windows operating system and SQL Server

Ensure all monitored SQL Server versions and their hosts support TLS 1.2.

Install all matching updates from Microsoft:

Ensure the ODBC Driver 13 for SQL Server is installed on all Spotlight Clients

Install ODBC Driver 13 if necessary.

Errors you might encounter if these requirements are not met

You may encounter connection errors like this from the Spotlight Client:

Windows pane

While monitoring a SQL Server connection you may see a similar message on the Monitored Server - SQL Server Connection Failure alarm where the collector has failed to connect to the SQL Server instance.